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STEMCELL Technologies Inc density gradient centrifugation lymphoprep solution
Density Gradient Centrifugation Lymphoprep Solution, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/density+gradient+solution+lymphoprep/lymphoprep/pm37166140-66-18-23
Average 90 stars, based on 1 article reviews
density gradient centrifugation lymphoprep solution - by Bioz Stars, 2026-10
90/100 stars

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Expressing:

Article Title: HLA-F transcriptional and protein differential expression according to its genetic polymorphisms.
Article Snippet: The average Ct was calculated with StepOne 2.1 software (Thermo Fisher Scientific), excluding Ct duplicates with a standard deviation above 0.5. .. 2.4.4 | HLA-F protein expression Peripheral blood mononuclear Cells (PBMC) cells were isolated from EDTA peripheral blood by density gradient centrifugation (Lymphoprep solution, StemCell) and cultured in FBS (Fetal Bovine Serum) supplemented RPMI overnight at 37 C before processing. ..

Isolation:

Article Title: HLA-F transcriptional and protein differential expression according to its genetic polymorphisms.
Article Snippet: The average Ct was calculated with StepOne 2.1 software (Thermo Fisher Scientific), excluding Ct duplicates with a standard deviation above 0.5. .. 2.4.4 | HLA-F protein expression Peripheral blood mononuclear Cells (PBMC) cells were isolated from EDTA peripheral blood by density gradient centrifugation (Lymphoprep solution, StemCell) and cultured in FBS (Fetal Bovine Serum) supplemented RPMI overnight at 37 C before processing. ..

Article Title: Mobilisation of HLA‐F on the surface of bronchial epithelial cells and platelets in asthmatic patients
Article Snippet: .. PBMC cells were isolated from EDTA peripheral blood collected 24 h before processing by density gradient centrifugation (Lymphoprep solution, StemCell) and cultured in FBS (Fetal Bovine Serum) supplemented RPMI overnight. ..

Article Title: Biodistribution of a Mitochondrial Metabolic Tracer, [ 18 F]F-AraG, in Healthy Volunteers
Article Snippet: .. Human PBMCs were isolated from whole blood using lymphoprep density gradient solution (Stemcell Technologies). .. CD8 + T cells were purified from PBMCs using CD8 MicroBeads (Miltenyi Biotec).

Article Title: HLA-H*02:07 Is a Membrane-Bound Ligand of Denisovan Origin That Protects against Lysis by Activated Immune Effectors.
Article Snippet: .. Effector PBMCs were isolated from EDTA peripheral blood samples by density gradient centrifugation (Lymphoprep solution; STEMCELL Technologies) and cultured in FBS-supplemented RPMI. .. PBMCs were activated by 100 IU/ml of recombinant human IL-2 (rhIL-2) for 24 h (Life Technologies).

Article Title: Immunosuppressive mechanisms of human bone marrow derived mesenchymal stromal cells in BALB/c host graft versus host disease murine models
Article Snippet: .. Mononuclear cells were isolated from the solution using Lymphoprep Density Gradient Medium. (Stemcell Technologies, Vancouver, BC). .. The isolate was then enriched for CD4+ cells with anti-mouse CD4 magnetic microbeads using the MidiMACS system (Miltenyi Biotech, Auburn, CA).

Article Title: The extracellular role of DNA damage repair protein APE1 in regulation of IL-6 expression
Article Snippet: .. Using the density gradient-based technique with Lymphoprep solution (Stem Cell Technologies), mononuclear cells were isolated from the whole blood. .. Monocytes and B cells were isolated from peripheral blood mononuclear cells by immune-magnetic negative selection with the Monocyte Isolation Kit II and the B Cell Isolation Kit II (Miltenyi Biotech), respectively, using the manufacturer's protocol.

Gradient Centrifugation:

Article Title: HLA-F transcriptional and protein differential expression according to its genetic polymorphisms.
Article Snippet: The average Ct was calculated with StepOne 2.1 software (Thermo Fisher Scientific), excluding Ct duplicates with a standard deviation above 0.5. .. 2.4.4 | HLA-F protein expression Peripheral blood mononuclear Cells (PBMC) cells were isolated from EDTA peripheral blood by density gradient centrifugation (Lymphoprep solution, StemCell) and cultured in FBS (Fetal Bovine Serum) supplemented RPMI overnight at 37 C before processing. ..

Article Title: Mobilisation of HLA‐F on the surface of bronchial epithelial cells and platelets in asthmatic patients
Article Snippet: .. PBMC cells were isolated from EDTA peripheral blood collected 24 h before processing by density gradient centrifugation (Lymphoprep solution, StemCell) and cultured in FBS (Fetal Bovine Serum) supplemented RPMI overnight. ..

Article Title: HLA-H*02:07 Is a Membrane-Bound Ligand of Denisovan Origin That Protects against Lysis by Activated Immune Effectors.
Article Snippet: .. Effector PBMCs were isolated from EDTA peripheral blood samples by density gradient centrifugation (Lymphoprep solution; STEMCELL Technologies) and cultured in FBS-supplemented RPMI. .. PBMCs were activated by 100 IU/ml of recombinant human IL-2 (rhIL-2) for 24 h (Life Technologies).

Cell Culture:

Article Title: HLA-F transcriptional and protein differential expression according to its genetic polymorphisms.
Article Snippet: The average Ct was calculated with StepOne 2.1 software (Thermo Fisher Scientific), excluding Ct duplicates with a standard deviation above 0.5. .. 2.4.4 | HLA-F protein expression Peripheral blood mononuclear Cells (PBMC) cells were isolated from EDTA peripheral blood by density gradient centrifugation (Lymphoprep solution, StemCell) and cultured in FBS (Fetal Bovine Serum) supplemented RPMI overnight at 37 C before processing. ..

Article Title: Mobilisation of HLA‐F on the surface of bronchial epithelial cells and platelets in asthmatic patients
Article Snippet: .. PBMC cells were isolated from EDTA peripheral blood collected 24 h before processing by density gradient centrifugation (Lymphoprep solution, StemCell) and cultured in FBS (Fetal Bovine Serum) supplemented RPMI overnight. ..

Article Title: HLA-H*02:07 Is a Membrane-Bound Ligand of Denisovan Origin That Protects against Lysis by Activated Immune Effectors.
Article Snippet: .. Effector PBMCs were isolated from EDTA peripheral blood samples by density gradient centrifugation (Lymphoprep solution; STEMCELL Technologies) and cultured in FBS-supplemented RPMI. .. PBMCs were activated by 100 IU/ml of recombinant human IL-2 (rhIL-2) for 24 h (Life Technologies).



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